FALL SEMESTER 2009 - BIOLOGY 3300 –
GENETICS
Lecture: CL 1008, T & TH 3:30 –
4:45 PM
Lab: all sections meet in SC 358; (05) T 5 – 7 PM, (06) TH 5 – 7 PM
Instructor: Dr. Dale Lynn Vogelien
Contact
Information: Office – SC 33; PH - (770)423 – 6507; email dvogelie@kennesaw.edu
Office
hours: M & W 4 – 6 PM, T & TH 10 AM - noon, others by appointment
Required and Recommended Course Materials – Course Description – Lecture Policy – Laboratory Policy – Exam Policy – Grading Policy -tentative Exam Schedule - tentative Lecture Schedule - tentative Laboratory Schedule (updated Sept. 24) - Policy Concerning Changes to the Syllabus - Course Withdrawal (updated Sept. 29) - Academic Integrity Policy - Inclement Weather Policy - Course Learning Objectives - Course Announcements & Materials (available through GeorgiaVIEW Vista; KSU netID and password required)
Genetics:
A Conceptual Approach, 3rd edition, by Benjamin A. Pierce
Laboratory safety glasses
Laboratory
Materials to Accompany Biol 3300, Fall Semester 2009 to Summer Semester 2010,
Fly Lab subscription (available from Biology Labs On-Line at http://www.biologylabsonline.com/)
Strongly Recommended (but optional)
Materials:
Solutions and Problem-Solving Megamanual to accompany Genetics: A Conceptual Approach
Laboratory notebook (permanent bound,
ruled)
COURSE DESCRIPTION (FROM THE
KSU UNDERGRADUATE CATALOG)
Principles
of genetics, including classical, molecular, population genetics and
cytogenetics. Importance of genetics as a foundation for other divisions of
biology is stressed. The laboratories will explore concepts in both
transmission and molecular genetics. Laboratory exercises will demonstrate the
principles of segregation and independent assortment. Sex linkage, epistatic
relationships, and the principles of human karyotyping will also be examined.
Exercises in molecular genetics will introduce students to the process of
recombinant DNA technology and will demonstrate the principles involved in
molecular separation techniques. Prerequisites: Biology 2107 & 2108; Chem
1211 & 1212. NOTE: A list of course learning objectives
is located at the end of the syllabus.
1.
Regular attendance is
essential for success in this class. If
you miss a lecture, YOU are responsible for obtaining all notes, announcements
and assignments. Course information
(lecture materials, announcements, copies of in-class quizzes, and take-home
quizzes) will be available online through GeorgiaVIEW Vista. Information
needed to access this page (e.g. user name and password) will be provided
during the first week of class.
2. Quizzes, in class and take-home, will be
given on a regular basis. All in-class quizzes will be announced the class
period before the quiz is to be given. The material to be tested will be
announced. Announcements will also be available online through GeorgiaVIEW Vista. In-class quizzes will be given
promptly at the beginning of lecture. No make-up quizzes will be given for
students arriving late. Take-home quizzes will be collected in class promptly
at the beginning of lecture on the date noted. Take-home quizzes turned in
after announcements/lecture has begun or at the end of class will NOT be
considered. In addition, quizzes emailed, left in my mailbox, left in the box
mounted on my office door, or left under my office door will NOT be accepted,
regardless of the time submitted. Note that 10 quizzes are planned for term,
and the 6 best grades will be used to determine the quiz grade. Each quiz is
worth 5 points.
3. The instructor may take roll periodically,
but attendance is not a factor in grade calculation.
1.
Laboratory is an integral part
of this course and attendance is required. Absence
from more than two laboratory periods
(unexcused absences - absences for which medical or legal documentation is not
provided) will result automatically in a final grade of "F" for the
term, regardless of other work.
2. Quizzes will be used to assess understanding of lab content and skills. Four quizzes (25 points each) are scheduled, and all of these count towards your final grade. Quizzes will be given during the first 20 minutes of lab (see Lab Schedule for dates). Students arriving late to lab will not be given additional time to complete a quiz.
3. Make-up lab quizzes are given for excused absences only (see item 6 below; absences for which medical or legal documentation can be provided). Given an unexcused absence, there is no make-up for a missed lab quiz. In this case students who miss a lab period during which a quiz was scheduled will receive a grade of 0 for the quiz. There is no substitute work/assignment that can be submitted in lieu of a missed lab quiz.
4.
Absence (unexcused) from a lab
exercise (a full lab period) results in a 1 point penalty on the corresponding
quiz.
5.
Attendance will be taken
promptly at the beginning of lab. Plan to arrive shortly before lab begins and
stay the ENTIRE lab period. Students that ARRIVE AFTER ATTENDANCE has been
recorded will be CONSIDERED LATE and will receive a LATE PENALTY of 1 point
that will be applied to the accompanying quiz. Students LEAVING LAB EARLY,
before the lab exercise is completed, will receive a 1 POINT PENALTY that will
be applied to the accompanying quiz.
6. Should you miss a lab due to a medical or legal
reason and official documentation is provided, this will be considered an
excused absence. When possible, notify the instructor of your absence prior to the designated lab time. Given
an excused absence, the penalties described in items 1 and 4 above will not be
applied to the corresponding lab quiz. However, and excused absence is not
without consequence. It is not possible for a student to perform a missed lab
activity outside of the regularly scheduled lab period.
1.
Exam format may consist of
multiple choice, true/false, short answer, and/or essay questions. Note:
genetics problems can be tested in all of these formats!
2.
Permission to miss an exam
will only be granted when a written medical or legal excuse is provided to me.
Students missing an exam must notify me before the scheduled exam
time (the only exception being an emergency). A make-up exam must be taken
within 1 week of the scheduled exam date. Failure to do so will result in a
grade of 0 for the exam.
3.
Four exams are scheduled (tentative
exam dates are provided below). Exams 1, 2, and 3 will be given during a
regularly scheduled lecture period. Exam 4 (the final exam) will be given
during finals period. The date and time are scheduled by the Registrar’s Office
and are not subject to change (refer to http://web.kennesaw.edu/registrar/fall2009_final_exams ).
4. The final exam will cover previously tested material and new material covered since exam 3. Actual coverage will be outlined in class.
5.
There are no planned extra-credit
projects. Do not expect a curve on individual exams, quizzes, or for the final
grade.
1.
The final course grade will be
based on the number of points earned out the total possible as follows:
4 Exams(100 pts each)
400
points
4 Lab Quizzes (25 pts. each)
100 points
6 Quizzes/Assignments (5 pts. Each) 30 points
530
total points
2.
A = 90 - 100 % of total
points; B = 80 - 89 %; C = 70 - 79 %; D = 60 - 69 %; F = below 60 %
3. If your grade on the comprehensive final is higher than the lowest grade earned for Exam 1, 2, or 3, it will be used to replace this lowest grade. In this case, the final exam grade will be counted twice. For example, consider exam 1, 2, and 3 scores of 50, 60, and 70, respectively, and a final exam score of 75. Since the final exam grade is higher than the lowest exam score of 50, it will replace this grade. The exam grades that would be used in the calculation of your course grade would be 75, 60, 70, and 75. If, however, your final exam grade is your lowest exam grade, then your grade will be calculated using all four exam grades.
Exam
1 September 22 (T)
Exam 2 October 20 (T)
Exam 3 November 17 (T)
Exam 4
December 8
(T)
|
Date |
Lecture |
Reading Assignment |
Study Questions |
|
8/18 |
Introduction to Genetics
(Chapt. 1); Chromosomes (Chapt. 2) |
1 (pp 5;11-12) |
5,
15-18, 22 |
|
8/20 & 8/25 |
Chromosomes
and Cellular Reproduction |
2 (pp 19-35) |
3, 5-11, 13-16, 18, 20, 22, 24-26, 28,29,
31, 33, 34 |
|
8/27 –
9/3 |
Genetic Variation (from
Chapt. 2); Basic Principles of Heredity (Chapt. 3) |
3 |
1-3, 9-11, 13, 14, 15a-b, 16-21, 23, 25-27,
29 - 35, 37-38 |
|
9/8 |
An Extension of Mendelian
Principles: Sex Linked Traits |
4(pp 81-87) |
23-25, 30-31, 35, 37-38 |
|
9/10 – 9/17 |
Additional Extensions
of Mendelian Principles |
5(pp 99-117 ½;
120-124) |
1, 2, 10-15, 17-18,23-25,
28, 31-32, 34-35, 42 |
|
9/22 |
EXAM 1 |
|
|
|
9/24 |
Pedigrees |
6
(pp 134-142) |
1, 3-6, 18–23 |
|
9/29 – 10/6 |
Linkage,
Recombination, and Eukaryotic Gene Mapping |
7 |
1-10, 13-34 |
|
10/8 |
Gender Determination
and Dosage Compensation |
4
|
3-9, 13-14, 16-18, 33-34, 41
|
|
10/13 & 10/15 |
Variation in Chromosome Number & Structure |
9 |
1-6, 9, 11-19, 21, 28, 31, 33-36, 38 |
|
10/20 |
EXAM 2 |
|
|
|
10/22 |
Nucleic Acid
Structure |
10 |
3-9, 11-12, 16-17, 22, 25-26, 28-30 |
|
10/27 |
Chromosome Structure
and Organization |
11 |
|
|
10/29 & 11/3 |
DNA Replication |
12 (pp 315-335) |
1-10, 15, 19, 23, 26 |
|
11/5 & 11/10 |
Transcription |
13 |
1-11, 14-19, 21, 23, 27-28, 30 |
|
11/12 |
RNA Processing |
14 (pp 373-386) |
4-8, 13-15, 19, 22, 24 |
|
11/17 |
EXAM 3 |
|
|
|
11/19 & 11/24 |
The Genetic Code and
Translation; Mutations |
15 (pp 399-401; 404-414 18 (pp 472-476) |
15: 6-12, 20-23, 25-28,
31 18: 2, 4, 16-20 |
|
12/1 |
Control of Gene
Expression |
16 (pp 426 – 441) |
1-6, 10-11, 14-16 |
|
12/3 |
Molecular Genetic
Analysis |
19 (pp 503-520) |
3-7, 9-10, 12, 30-31 |
|
12/8 |
Final Exam (3:30 – 5:30 PM) |
Comprehensive |
|
|
Date section 05/06 |
Activity |
|
Aug. 18/20 |
NO LAB |
|
Aug. 25/27 |
Lab
1: Simulation of Chromosome Behavior |
|
Sept. 1/3 |
Lab
2: Patterns of Inheritance in Maize |
|
Sept. 8/10 |
NO LAB (Week of Labor
Day |
|
Sept. 15/17 |
LAB QUIZ 1 (covers labs 1 & 2) |
|
Sept. 22/24 |
|
|
Sept.
29/Oct. 1 |
Lab
4: Characterization of Drosophila Mutations Using Virtual FlyLab |
|
Oct. 6/8 |
LAB QUIZ 2 (covers labs 3 & 4) Lab 5: Determination of Linkage Relationships Using Virtual FlyLab
|
|
Oct. 13/15 |
Lab 6: Molecular
Genetics: Hemoglobin Analysis Using Gel Electrophoresis |
|
Oct. 20/22 |
LAB QUIZ 3 (covers labs 5 and 6) |
|
Oct. 27/29 |
Lab
7: Transformation of E. coli |
|
Nov.
3/5 |
Complete Lab 7 Lab 8: Analysis of DNA by Gel Electrophoresis (perform restriction digestions and prepare gels) |
|
Nov. 10/12 |
Lab
8 continued: Analysis of DNA by Gel Electrophoresis (perform electrophoretic analysis
of digests) |
|
Nov.
17/19 |
Lab Discussion |
|
Nov. 24/26 |
NO LAB (Fall Break ) |
|
Dec. 1/3 |
Lab QUIZ 4 (covers labs 7 & 8); Course Evaluations;
Discussion of Final Exam
|
*Quizzes will be given during the first 20 minutes of lab.
Students arriving late to lab will not be given additional time to complete a
quiz. Students missing a lab quiz will receive a grade of 0 for the quiz.
Quizzes are given only during lab – never outside of lab.
POLICY
CONCERNING CHANGES TO THE SYLLABUS
Any changes in this syllabus will be announced
in class and posted online at the course homepage accessed through GeorgiaVIEW Vista. In addition, this course syllabus will be modified to reflect the change(s), and the date of modification
noted in the headings section (next to the associated section).
If the University
is CLOSED, there will be no classes or labs. Otherwise lecture and lab classes will
meet as scheduled. For the official status of the university check the KSU
website: http://www.kennesaw.edu/ and
click on “Campus Advisories” under Online Resources.
COURSE
WITHDRAWAL
Students may withdraw from one or more
courses anytime before the last three weeks of the semester. However, as of
fall 2004, students will be allowed a maximum of eight total withdrawals if
they enter KSU as a freshman. Transfer students will be allowed one withdrawal
per fifteen credit hours attempted, for a maximum of eight. Students who choose
to pursue a second degree at KSU will be allowed two additional withdrawals.
Students who entered KSU before the fall of 2004 will be allowed one withdrawal
per fifteen credit hours attempted for a maximum of eight after the institution
of this policy. To withdraw from a course at KSU, a student must withdraw
online at www.kennesaw.edu through Owl Express (under Online
Resources for Students). Students who officially withdraw from courses on or
before 11:45 PM October 19 will receive a "W" in those courses
and receive no credit. They will not however suffer any academic penalty.
Students who officially withdraw after 11:45 PM October 19 will receive
a "WF," which will be counted as an "F" in the calculation
of their grade point average. The only exceptions to these withdrawal
regulations will be for instances involving unusual circumstances, which are
fully documented. Students may appeal to the academic standing committee for
consideration of unusual circumstances. Students who simply stop attending
classes without officially withdrawing usually are assigned failing grades.
KSU’s
ACADEMIC INTEGRITY POLICY
Every KSU student is responsible for upholding the provisions
of the Student Code of Conduct, as published in the Undergraduate and Graduate
Catalogs. Section II of the Student Code of Conduct addresses the University’s
policy on academic honesty, including provisions regarding plagiarism and
cheating, unauthorized access to University materials,
misrepresentation/falsification of University records or academic work,
malicious removal, retention, or destruction of library materials,
malicious/intentional misuse of computer facilities and/or services, and misuse
of student identification cards. Incidents of alleged academic misconduct will
be handled through the established procedures of the University Judiciary
Program, which includes either an “informal” resolution by a faculty member,
resulting in a grade adjustment, or a formal hearing procedure, which may
subject a student to the Code of Conduct’s minimum one semester suspension
requirement. Please refer to
the KSU Undergraduate Catalog (select Statement of Student Rights and Responsabililties; select Student Code of conduct) for more
information.
1)
Cell
and Organism Reproduction:
Students will be able to: describe cellular and chromosomal events that occur
during the eukaryotic cell cycle and gamete formation; describe chromosome
behavior and changes in chromosome structure and number as a cell progresses
through a cell cycle, meiosis I and meiosis II; explain how meiosis and random
fertilization contribute to genetic variation in sexually reproducing
organisms.
2)
Principles
of Heredity: Students will
be able to: explain Mendel’s principles of inheritance and apply these to
problems of inheritance; describe the different forms of inheritance patterns and
identify these in genetic data; use
and interpret probabilities and statistics in the gathering, predicting, and
analysis of genetic data; describe various types of genetic crosses and
indicate when/why they would be used by a geneticist; explain more complex modes
of inheritance and how sex influences the inheritance and expression of genes
(e.g. sex-influenced traits, cytoplasmic inheritance, genomic imprinting); use
this information in predicting genetic outcomes and the analysis of genetic
data.
3)
Pedigrees: Students will be able to: apply principles
of heredity and their understanding of various modes of inheritance in
assessment of pedigrees, identifying genotypes of family members, concluding
the mode of inheritance for a trait, and predicting mating outcomes.
4)
Eukaryotic
Gene Mapping: Students
will be able to: compare the effect of linked genes to those that assort
independently on genetic variation and assess if genes are linked or located on
separate chromosomes; explain how crossing over produces recombination and use
recombination frequencies to construct a map linked genes; describe some of the
methods that can be used to place a gene on a particular chromosome (e.g. FISH,
somatic-cell hybridization).
5)
Chromosome
Variation and Structure: Students
will be able to: describe
and recognize a variety of abnormalities in chromosome structure and number and
explain how these anomalies arise and are detected; explain the molecular structure of
chromosomes as it relates to storage, gene expression, and sequence function.
6)
Nucleic
Acid Structure: Students
will be able to describe early studies that led to DNA as the genetic material
and/or interpret results from these studies; describe the molecular structure
of DNA and RNA and indicate similarities and differences; recognize the major
differences in structure between B, A, and Z DNA and the conditions under which
each exists.
7)
DNA
Replication: Students will
be able to: describe the historic experiment that demonstrated DNA replication
follows a semi-conservative model; describe the process of DNA replication in
prokaryotes at the biochemical level; explain how proofreading and repair is
accomplished during DNA synthesis; describe how DNA is replicated in viruses,
plasmids, and eukaryotes and identify similarities and differences between
these and replication in prokaryotes.
8)
Gene
Expression: Students will
be able to: describe at the biochemical level the events that occur to go from
gene to phenotype; identify different types of RNA, note their properties, how
they are processed to yield a functional form, and their function in gene
expression; recognize the importance of regulating gene expression in
prokaryotes and eukaryotes and describe the levels at which gene expression is
controlled; explain the structure and function of operons.
9) Mutations: Students will be able to define and identify the various
types of mutations that occur at the DNA and protein levels and explain and
recognize the relationship between mutations and new alleles.
10) Molecular Genetic
Analysis: Students
will be able to explain major methods and techniques used in molecular genetics
to isolate, recombine, amplify, find and study genes of interest.